Abstract: The response spectrum theory widely applied in earthquake engineering was employed to investigate blasting seismic waves induced by tunnel excavation. After numerical differential calculating for blasting vibration velocity data, an original acceleration wave curve involving with high-frequency noises was obtained. When the high-frequency noise was cleared up by wavelet transform, an accurate and clear blasting vibration acceleration curve was obtained. And then the acceleration data were used to calculate the response spectrum of blasting vibration. Response spectrum analysis of blasting vibration induced by tunnel excavation was performed with a vast amount of monitoring data in diferent projects. The result shows that response spectrum of blasting vibration induced by tunnel excavation has some same charactors. Response spectrum curves generally have a distinct peak point. At the peak point, the period is less than 100 ms. In addition, based on coordinating and suming up a great deal of response spectrum curves induced by tunnel excavation, the design response spectrum curves were presented. This research can provide a guide for studying the blasting seismic effect.