Effective improvement of retinal pathological injury and autophagy changes induced by explosive eye blast injury by protective goggles

CHAO Wangshu; FEI Xiaowei; LI Weiping; DOU Yaʼnan; WANG Junlong; HE Xin; LYU Weihao; WU Xiuquan; CHEN Hongqing; CHEN Leiying; MA Tian; FEI Zhou; ZHUANG Zhuo; KANG Yue; FEI Fei

doi:10.11883/bzycj-2024-0257

Volume 44 Issue 12

Dec. 2024

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Article Navigation > Explosion And Shock Waves > 2024 > 44(12): 121441

CHAO Wangshu, FEI Xiaowei, LI Weiping, DOU Yaʼnan, WANG Junlong, HE Xin, LYU Weihao, WU Xiuquan, CHEN Hongqing, CHEN Leiying, MA Tian, FEI Zhou, ZHUANG Zhuo, KANG Yue, FEI Fei. Effective improvement of retinal pathological injury and autophagy changes induced by explosive eye blast injury by protective goggles[J]. Explosion And Shock Waves, 2024, 44(12): 121441. doi: 10.11883/bzycj-2024-0257

Citation:

CHAO Wangshu, FEI Xiaowei, LI Weiping, DOU Yaʼnan, WANG Junlong, HE Xin, LYU Weihao, WU Xiuquan, CHEN Hongqing, CHEN Leiying, MA Tian, FEI Zhou, ZHUANG Zhuo, KANG Yue, FEI Fei. Effective improvement of retinal pathological injury and autophagy changes induced by explosive eye blast injury by protective goggles[J]. Explosion And Shock Waves, 2024, 44(12): 121441. doi: 10.11883/bzycj-2024-0257

Citation:

CHAO Wangshu, FEI Xiaowei, LI Weiping, DOU Yaʼnan, WANG Junlong, HE Xin, LYU Weihao, WU Xiuquan, CHEN Hongqing, CHEN Leiying, MA Tian, FEI Zhou, ZHUANG Zhuo, KANG Yue, FEI Fei. Effective improvement of retinal pathological injury and autophagy changes induced by explosive eye blast injury by protective goggles[J]. Explosion And Shock Waves, 2024, 44(12): 121441. doi: 10.11883/bzycj-2024-0257

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Effective improvement of retinal pathological injury and autophagy changes induced by explosive eye blast injury by protective goggles

doi: 10.11883/bzycj-2024-0257

1.
Basic Medical Science Academy, Air Force Medical University, Xiʼan 710032, Shaanxi, China
2.
Xijing Hospital, Air Force Medical University, Xiʼan 710032, Shaanxi, China
3.
Institute of Systems Engineering, Academy of Military Sciences, Beijing 100010, China
4.
School of Aerospace Engineering, Tsinghua University, Beijing 100084, China

Received Date: 2024-07-25
Rev Recd Date: 2024-10-24

Available Online: 2024-10-29

Publish Date: 2024-12-01

Abstract

Abstract

We firstly verified the protective performance of eye equipment (goggles) based on a head dynamic test system and shock tube and field live blast test environments. The results show that goggles have better protective performance and suggest that duty personnel should be equipped with goggles that have combined functions of anti-ultraviolet, anti-glare, anti-smoke and anti-fragmentation in to improve eye protection capabilities. After that, we investigated the tissue damage and functional impairment changes after explosive eye blast injury, and the protective effect and mechanism of the goggles for animal experimental version. This may provide a theoretical basis for prevention and treatment, and also have important implications for the design and improvement of protective goggles. Beagles and C57 mice were used for related animal experiments, and the changes in retinal layer thickness and cell apoptosis were observed after blast injury by HE, Tunel, Nissl staining, visual electrophysiology detection and other methods. It was found that with the increase of blast intensity and the extension of time after explosion, both the degree of retinal injury and cell apoptosis increased, among which the ganglion cell layer and photoreceptor inner and outer segments suffered the most severe damage. Further research on molecular changes indicates that the expression levels of autophagy-related regulatory proteins SQSTM1/p62 (P < 0.0001) and LC3-Ⅱ (P = 0.8437), as well as LC3-Ⅰ (P = 0.003), are significantly increased, suggesting that retinal damage is, to some extent, induced by autophagic mechanisms. The protective goggles could effectively reduce the damage of blast wave to retina, protect the structural integrity of retinal nerve fiber layer, inner and outer nuclear layer, ganglion cell layer and photoreceptor inner and outer segments. At the same time, compared with that of other groups, the difference in retinal layer thickness and cell apoptosis was most significant in the 3.5 MPa group, suggesting that the glasses played the maximum protective effect at this intensity, which may be related to the reduction in the retinal autophagy.
- beagles,
- explosive eye blast injury,
- protective goggles,
- retina,
- mitochondria,
- autophagy

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References(25)

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