An auditory damage model for inner ears of miniature pigs based on free-field explosion

XUE Songbo; XIANG Shuyi; ZHAO Yang; DU Zhibo; WANG Xinghao; LI Yifeng; ZHANG Jiarui; FEI Zhou; TIAN Xu; GAO Zhiqiang; ZHUANG Zhuo; LIU Zhanli; FENG Guodong

doi:10.11883/bzycj-2024-0256

Volume 44 Issue 12

Dec. 2024

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Article Navigation > Explosion And Shock Waves > 2024 > 44(12): 121432

XUE Songbo, XIANG Shuyi, ZHAO Yang, DU Zhibo, WANG Xinghao, LI Yifeng, ZHANG Jiarui, FEI Zhou, TIAN Xu, GAO Zhiqiang, ZHUANG Zhuo, LIU Zhanli, FENG Guodong. An auditory damage model for inner ears of miniature pigs based on free-field explosion[J]. Explosion And Shock Waves, 2024, 44(12): 121432. doi: 10.11883/bzycj-2024-0256

Citation:

XUE Songbo, XIANG Shuyi, ZHAO Yang, DU Zhibo, WANG Xinghao, LI Yifeng, ZHANG Jiarui, FEI Zhou, TIAN Xu, GAO Zhiqiang, ZHUANG Zhuo, LIU Zhanli, FENG Guodong. An auditory damage model for inner ears of miniature pigs based on free-field explosion[J]. Explosion And Shock Waves, 2024, 44(12): 121432. doi: 10.11883/bzycj-2024-0256

Citation:

XUE Songbo, XIANG Shuyi, ZHAO Yang, DU Zhibo, WANG Xinghao, LI Yifeng, ZHANG Jiarui, FEI Zhou, TIAN Xu, GAO Zhiqiang, ZHUANG Zhuo, LIU Zhanli, FENG Guodong. An auditory damage model for inner ears of miniature pigs based on free-field explosion[J]. Explosion And Shock Waves, 2024, 44(12): 121432. doi: 10.11883/bzycj-2024-0256

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An auditory damage model for inner ears of miniature pigs based on free-field explosion

doi: 10.11883/bzycj-2024-0256

1.
Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China
2.
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China
3.
School of Aerospace Engineering, Tsinghua University, Beijing 100084, China
4.
Xijing Hospital, Air Force Medical University, Xi’an 710032, Shaanxi, China

Received Date: 2024-07-25
Rev Recd Date: 2024-10-24

Available Online: 2024-10-25

Publish Date: 2024-12-01

Abstract

Abstract

A realistic blast injury model was developed for simulating auditory damage in the inner ears of miniature pigs under controlled explosion conditions to investigate the impact of varying blast shockwave pressures on auditory impairment. Fourteen healthy miniature pigs were selected and underwent auditory brainstem response (ABR) testing prior to exposure to explosions. A free-field explosion platform was constructed utilizing 1.9 kg and 8.0 kg of TNT, with the explosive source 1.8 meters above the ground. The pigs were securely fixed in protective devices, exposing only their head, and placed at varying distances from the blast source. Peak shockwave pressures were measured, and immediate mortality rates were calculated accordingly. Post-explosion ABR tests were conducted, followed by examination of cochlear tissues using scanning electron microscopy to analyze hair cell damage. Shockwave peak pressures ranged from 96.3 kPa to 628.3 kPa over a distance range of 1.8 m to 3.8 m, with pressure decreasing as distance increased. At a distance of 2.6 m, a peak pressure of 628.3 kPa resulted in a mortality ratio of 50%. ABR threshold comparisons before and after the explosion revealed significant increases across all tested frequencies (P < 0.05), with the most notable changes at a frequency of 4 kHz. Scanning electron microscopy analysis demonstrated that inner hair cells exhibited greater susceptibility to damage compared to outer hair cells, with higher shockwave pressure leading to more sever damage. Blast shockwaves caused substantial auditory system damage to miniature pigs as evidenced by elevated ABR thresholds and destruction of cochlear hair cell. Inner hair cells proved more vulnerable to blast shockwaves. The established model can provide a valuable experimental foundation for further studies on blast injury mechanisms and protective strategies.
- blast injury,
- miniature pig,
- inner ear auditory function,
- cochlear injury

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References(27)

References

[1]	SULLIVAN E V. War-related PTSD, blast injury, and anosognosia [J]. Neuropsychology Review, 2012, 22(1): 1–2. DOI: 10.1007/s11065-012-9188-z.
[2]	MCEVOY C B, CRABTREE A, POWELL J R, et al. Cumulative blast exposure estimate model for special operations forces combat soldiers [J]. Journal of Neurotrauma, 2023, 40(3/4): 318–325. DOI: 10.1089/neu.2022.0075.
[3]	PAIK C B, PEI M, OGHALAI J S. Review of blast noise and the auditory system [J]. Hearing Research, 2022, 425: 108459. DOI: 10.1016/j.heares.2022.108459.
[4]	MIZUTARI K. Blast-induced hearing loss [J]. Journal of Zhejiang University: Science B, 2019, 20(2): 111–115. DOI: 10.1631/jzus.B1700051.
[5]	刁明芳. 爆震伤对听觉系统的损害与影响 [J]. 中国耳鼻咽喉颅底外科杂志, 2016, 22(3): 254–256. DOI: 10.11798/j.issn.1007-1520.201603022.
[6]	PARK W J, MOON J D. Changes in the mean hearing threshold levels in military aircraft maintenance conscripts [J]. Archives of Environmental and Occupational Health, 2016, 71(6): 347–352. DOI: 10.1080/19338244.2015.1136588.
[7]	NIWA K, MIZUTARI K, MATSUI T, et al. Pathophysiology of the inner ear after blast injury caused by laser-induced shock wave [J]. Scientific Reports, 2016, 6: 31754. DOI: 10.1038/srep31754.
[8]	CHO S I, GAO S S, XIA A P, et al. Mechanisms of hearing loss after blast injury to the ear [J]. PLoS One, 2013, 8(7): e67618. DOI: 10.1371/journal.pone.0067618.
[9]	谭君武, 彭洪. 传统方法联合银杏叶注射液治疗爆震性聋的临床分析 [J]. 中华劳动卫生职业病杂志, 2015, 33(4): 279–281. DOI: 10.3760/cma.j.issn.1001-9391.2015.04.013. TAN J W, PENG H. Clinical analysis of Ginkgo biloba injection combined with traditional therapy in treatment of explosive deafness [J]. Chinese Journal of Industrial Hygiene and Occupational Diseases, 2015, 33(4): 279–281. DOI: 10.3760/cma.j.issn.1001-9391.2015.04.013.
[10]	NAERT G, PASDELOU M P, LE PRELL C G. Use of the guinea pig in studies on the development and prevention of acquired sensorineural hearing loss, with an emphasis on noise [J]. The Journal of the Acoustical Society of America, 2019, 146(5): 3743–3769. DOI: 10.1121/1.5132711.
[11]	ARUN P, WILDER D M, EKEN O, et al. Long-term effects of blast exposure: a functional study in rats using an advanced blast simulator [J]. Journal of Neurotrauma, 2020, 37(4): 647–655. DOI: 10.1089/neu.2019.6591.
[12]	JIANG S Y, WELCH P, SANDERS S, et al. Mitigation of hearing damage after repeated blast exposures in animal model of chinchilla [J]. Journal of the Association for Research in Otolaryngology, 2022, 23(5): 603–616. DOI: 10.1007/s10162-022-00862-2.
[13]	DAHLQUIST A, ELANDER DEGERSTEDT L, VON OELREICH E, et al. Blast polytrauma with hemodynamic shock, hypothermia, hypoventilation and systemic inflammatory response: description of a new porcine model [J]. European Journal of Trauma and Emergency Surgery, 2022, 48(1): 401–409. DOI: 10.1007/s00068-020-01476-0.
[14]	GURR A, STARK T, PROBST G, et al. The temporal bone of lamb and pig as an alternative in ENT-education [J]. Laryngorhinootologie, 2010, 89(1): 17–24. DOI: 10.1055/s-0029-1224158.
[15]	JIANG S Y, SANDERS S, GAN R Z. Hearing protection and damage mitigation in Chinchillas exposed to repeated low-intensity blasts [J]. Hearing Research, 2023, 429: 108703. DOI: 10.1016/j.heares.2023.108703.
[16]	ARTERO-GUERRERO J, PERNAS-SÁNCHEZ J, TEIXEIRA-DIAS F. Blast wave dynamics: the influence of the shape of the explosive [J]. Journal of Hazardous Materials, 2017, 331: 189–199. DOI: 10.1016/j.jhazmat.2017.02.035.
[17]	DEWEY J M. An interface to provide the physical properties of the blast wave from a free-field TNT explosion [J]. Shock Waves, 2022, 32(4): 383–390. DOI: 10.1007/s00193-022-01076-4.
[18]	DION G R, MILLER C L, O’CONNOR P D, et al. Correlation of otologic complaints in soldiers with speech disorders after traumatic brain injury [J]. Journal of Voice, 2014, 28(1): 88–91. DOI: 10.1016/j.jvoice.2013.08.005.
[19]	CAMPBELL K, CLAUSSEN A, MEECH R, et al. D-methionine (D-met) significantly rescues noise-induced earing loss: timing studies [J]. Hearing Research, 2011, 282(1/2): 138–144. DOI: 10.1016/j.heares.2011.08.003.
[20]	SHAO N N, JIANG S Y, YOUNGER D, et al. Central and peripheral auditory abnormalities in chinchilla animal model of blast-injury [J]. Hearing Research, 2021, 407: 108273. DOI: 10.1016/j.heares.2021.108273.
[21]	CAMPBELL K C M, MEECH R P, KLEMENS J J, et al. Prevention of noise- and drug-induced hearing loss with D-methionine [J]. Hearing Research, 2007, 226(1/2): 92–103. DOI: 10.1016/j.heares.2006.11.012.
[22]	EWERT D L, LU J Z, LI W, et al. Antioxidant treatment reduces blast-induced cochlear damage and hearing loss [J]. Hearing Research, 2012, 285(1/2): 29–39. DOI: 10.1016/j.heares.2012.01.013.
[23]	KIM J, XIA A P, GRILLET N, et al. Osmotic stabilization prevents cochlear synaptopathy after blast trauma [J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(21): E4853–E4860. DOI: 10.1073/pnas.1720121115.
[24]	LIU C C, GAO S S, YUAN T, et al. Biophysical mechanisms underlying outer hair cell loss associated with a shortened tectorial membrane [J]. Journal of the Association for Research in Otolaryngology, 2011, 12(5): 577–594. DOI: 10.1007/s10162-011-0269-0.
[25]	CHEN T, SMITH K, JIANG S Y, et al. Progressive hearing damage after exposure to repeated low-intensity blasts in chinchillas [J]. Hearing Research, 2019, 378: 33–42. DOI: 10.1016/j.heares.2019.01.010.
[26]	HUANG X Y, XIA B C, CHANG L J, et al. Experimental study on intracranial pressure and biomechanical response in rats under the blast wave [J]. Journal of Neurotrauma, 2024, 41(5/6): 671–684. DOI: 10.1089/neu.2022.0229.
[27]	ANDERSON D A, ARGO T F, GREENE N T. Occluded insertion loss from intracochlear pressure measurements during acoustic shock wave exposure [J]. Hearing Research, 2023, 428: 108669. DOI: 10.1016/j.heares.2022.108669.